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Biofabrication of streptomycin-conjugated calcium phosphate nanoparticles using red ginseng extract and investigation of their antibacterial potential.
- Source :
-
PLoS ONE . 6/10/2019, Vol. 14 Issue 6, p1-17. 17p. - Publication Year :
- 2019
-
Abstract
- Conjugation of nanoparticles (NPs) with antibiotics for treating multidrug resistant pathogens has been enormously studied now a days. In the current investigation, calcium phosphate (CaP) NPs were produced by co-precipitation using red ginseng extract as the reducing agent and were conjugated to the antibiotic streptomycin to form streptomycin-conjugated NPs (CPG-S NPs). The CPG-S NPs antibacterial activity was evaluated in this study against eight plant and five foodborne pathogenic bacteria. The synthesized CPG-S NPs were characterized by UV-VIS spectroscopy, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectroscopy, X-ray powder diffraction, and thermogravimetric and differential thermogravimetric analysis. CPG-S NPs exhibited promising antibacterial activity against all eight plant pathogenic bacteria and three of the five foodborne pathogenic bacteria tested; the diameter of inhibition zones ranged between 9.74–16.95 mm and 9.82–15.84 mm, respectively. CPG-S NPs displayed 50–100 μg/mL of minimum inhibitory concentration and 100 μg/mL of minimum bactericidal concentration against the plant and foodborne pathogenic bacterial strains, respectively. Furthermore, the SEM image of bacteria treated with CPG-S NPs displayed cells with a ruptured cell wall and fewer cells compared to the SEM image of untreated control bacteria displaying uniform and intact cells. SEM confirmed that CPG-S NPs degraded the bacterial cell wall and membrane resulting in lysed bacterial cells. In conclusion, the results suggest that CPG-S NPs could be effectively utilized in formulating drugs to treat bacterial plant or dental diseases and in manufacturing dental products such as toothpaste, mouthwashes, and artificial teeth. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 14
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- PLoS ONE
- Publication Type :
- Academic Journal
- Accession number :
- 136887203
- Full Text :
- https://doi.org/10.1371/journal.pone.0217318