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Covalent immobilization and characterization of penicillin G acylase on amino and GO functionalized magnetic Ni0.5Zn0.5Fe2O4@SiO2 nanocomposite prepared via a novel rapid-combustion process.
- Source :
-
International Journal of Biological Macromolecules . Aug2019, Vol. 134, p507-515. 9p. - Publication Year :
- 2019
-
Abstract
- Magnetic Ni 0.5 Zn 0.5 Fe 2 O 4 @SiO 2 nanocomposite was prepared via the rapid combustion process, and its surface was modified to obtain amino-functionalized magnetic Ni 0.5 Zn 0.5 Fe 2 O 4 @SiO 2 -NH 2 nanocomposite. The modified nanocomposite was loaded on graphene oxide (GO), on which penicillin G acylase (PGA) was covalently immobilized. The structure for docking was visualized between PGA and penicillin G using the PyMol program, which revealed the configuration of the active site. Selections of immobilization conditions including immobilization concentration and time of fixation, were explored. The catalytic performance of the immobilized PGA was characterized. The immobilized and free PGA had the highest activity at pH 8.0 and 45 °C. Compared with the activity of the free PGA, the activity of the immobilized PGA was affected less by pH and temperature. The immobilized PGA exhibited the high-effective activity and good stability. V max and K m of immobilized PGA were 0.8123 mol·min−1 and 0.0399 mol·L−1, respectively. Free PGA's V max and K m were 0.6854 mol·min−1 and 0.0328 mol·L−1. Immobilized PGA remained >70% in relative activity after 9 successive cycles. • Magnetic Ni 0.5 Zn 0.5 Fe 2 O 4 @SiO 2 nanocomposite was prepared via a rapid combustion process. • Penicillin G acylase (PGA) was immobilized onto Ni 0.5 Zn 0.5 Fe 2 O 4 @SiO 2 -NH-GO composite by covalent bond. • The structure for docking was visualized Pymol between PGA and penicillin G potassium salt using the program. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PENICILLIN G
*GRAPHENE oxide
*COVALENT bonds
*POLYPYRROLE
*POTASSIUM salts
Subjects
Details
- Language :
- English
- ISSN :
- 01418130
- Volume :
- 134
- Database :
- Academic Search Index
- Journal :
- International Journal of Biological Macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 137128084
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2019.05.066