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DNAzyme-Functionalized R-Phycoerythrin as a Cost-Effective and Environment-Friendly Fluorescent Biosensor for Aqueous Pb2+ Detection.
- Source :
-
Sensors (14248220) . Jun2019, Vol. 19 Issue 12, p2732. 1p. - Publication Year :
- 2019
-
Abstract
- The sensitive detection of Pb2+ is of significant importance for food safety, environmental monitoring, and human health care. To this end, a novel fluorescent biosensor, DNAzyme-functionalized R-phycoerythrin (DNAzyme-R-PE), was presented for Pb2+ analysis. The biosensor was prepared via the immobilization of Iowa Black® FQ-modified DNAzyme–substrate complex onto the surface of SPDP-functionalized R-PE. The biosensor produced a minimal fluorescence signal in the absence of Pb2+. However, Pb2+ recognition can induce the cleavage of substrate, resulting in a fluorescence restoration of R-PE. The fluorescence changes were used to measure sensitively Pb2+ and the limit of detection was 0.16 nM with a linear range from 0.5–75 nM. Furthermore, the proposed biosensor showed excellent selectivity towards Pb2+ even in the presence of other metal ions interferences and was demonstrated to successfully determine Pb2+ in spiked lake water samples. [ABSTRACT FROM AUTHOR]
- Subjects :
- *BIOSENSORS
*FLUORESCENCE resonance energy transfer
*ENVIRONMENTAL monitoring
Subjects
Details
- Language :
- English
- ISSN :
- 14248220
- Volume :
- 19
- Issue :
- 12
- Database :
- Academic Search Index
- Journal :
- Sensors (14248220)
- Publication Type :
- Academic Journal
- Accession number :
- 137272635
- Full Text :
- https://doi.org/10.3390/s19122732