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Recombinant Antibodies against Mycolactone.

Authors :
Naranjo, Leslie
Ferrara, Fortunato
Blanchard, Nicolas
Demangel, Caroline
D'Angelo, Sara
Erasmus, M. Frank
Teixeira, Andre A.
Bradbury, Andrew R.M.
Source :
Toxins. Jun2019, Vol. 11 Issue 6, p346-346. 1p.
Publication Year :
2019

Abstract

In the past, it has proved challenging to generate antibodies against mycolactone, the primary lipidic toxin A of Mycobacterium ulcerans causing Buruli ulcer, due to its immunosuppressive properties. Here we show that in vitro display, comprising both phage and yeast display, can be used to select antibodies recognizing mycolactone from a large human naïve phage antibody library. Ten different antibodies were isolated, and hundreds more identified by next generation sequencing. These results indicate the value of in vitro display methods to generate antibodies against difficult antigenic targets such as toxins, which cannot be used for immunization unless inactivated by structural modification. The possibility to easily generate anti-mycolactone antibodies is an exciting prospect for the development of rapid and simple diagnostic/detection methods. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20726651
Volume :
11
Issue :
6
Database :
Academic Search Index
Journal :
Toxins
Publication Type :
Academic Journal
Accession number :
137289452
Full Text :
https://doi.org/10.3390/toxins11060346