Valorization of food-waste hydrolysate by Lentibacillus salarius NS12IITR for the production of branched chain fatty acid enriched lipid with potential application as a feedstock for improved biodiesel.

• Production of branched-chain fatty acids enriched lipid (81% of total FA) without any supplementation in medium. • Use of food-waste hydrolysate as substrate. • Scalable cell lysis method followed by solvent extraction for lipid isolation. • Energy efficient lipid isolation method. Oxidation stability and cold flow properties of biodiesel can be improved by using lipid with enriched branched-chain fatty acid (BCFA) as a feedstock. A halophilic bacterium was utilized for the production of BCFA enriched lipid from acid hydrolysate of food-waste. The maximum reducing sugar obtained by hydrolysis of wheat bran, rice bran, mango peel, and orange peel were 64.52 ± 0.57, 38.7 ± 0.58, 55.64 ± 1.14, 36.29 ± 0.54 g/L, respectively. On assessing these hydrolysates as feedstock for growth of halophilic bacterium Lentibacillus salarius NS12IITR at 10 g/L reducing sugar concentration, wheat bran hydrolysate was found to be best in-terms of sugar consumption (92%), lipid production (0.70 ± 0.029 g/L) and maximum branched-chain fatty acid methyl ester (FAME) (81 ± 4.72% of total FAME). At 20 g/L of reducing sugar concentration of wheat bran hydrolysate, the biomass and lipid yields were almost doubled. Efficient lipid extraction from cell, involving thermolysis at 85 °C and pH 2 along with osmotic shock resulted in isolation of 69% of total lipid. [ABSTRACT FROM AUTHOR]