Labile carbon matters more than temperature for enzyme activity in paddy soil.

Global warming increases belowground carbon (C) input as plant litterfall, root biomass and rhizodeposition, which influences the stocks and dynamics of soil organic matter. To clarify the effects of labile C availability (biochemical factor) and temperature (environmental factor) on enzyme activities, we incubated typical paddy soil for 75 d at four temperatures (5, 15, 25, and 35 °C) under anaerobic conditions. Acetate was used as the source of labile C and methane. The potential activities of three hydrolases (β -glucosidase, chitinase, and xylanase) were analysed on days 3, 15, and 75 after acetate addition. Activity of β -glucosidase and chitinase in soil without acetate addition was 2.1–2.7 times higher than that with acetate. Xylanase activity increased with temperature and incubation period. The enzymes involved in the C cycle were sensitive to temperature, whereas chitinase (responsible for N cycle) activity became temperature sensitive only after acetate addition (Q 10 - V max ≥ 1). Organic C mineralisation (CO 2 release) was more sensitive at low temperature with Q 10 values 1.1–3.4 times higher at 5–15 °C than at 25–35 °C. The Q 10 values for methane (CH 4) emission were 2.8–13.5 times higher at 5–15 °C than at 25–35 °C. Organic matter decomposition in paddy soil was more sensitive to temperature (Q 10 of CO 2 and CH 4 emission ≥ 1) than enzyme activities. Comparison of abiotic (temperature) and biochemical (C availability) effects indicated that warming has limited effects on hydrolase activities in paddy soil. The increase in labile C remarkably stimulated microbial activity and soil organic matter turnover. We conclude that: i) enzyme activities are more sensitive to C addition than to temperature; ii) and SOM decomposition is accelerated by both C input and warming, especially at low temperatures. Image 1 • Labile C affects the response of extracellular enzyme activities to soil warming. • The higher the labile C content, the less enzymes are produced by soil microorganisms. • Labile C addition affects microbial biomass and induces enzymatic N mining from SOM. • Acetate input increases temperature sensitivity of CO 2 and CH 4 emission below 15 °C. [ABSTRACT FROM AUTHOR]