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Determination of ceftriaxone concentration in human cerebrospinal fluid by high-performance liquid chromatography with UV detection.
- Source :
-
Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences . Aug2019, Vol. 1124, p161-164. 4p. - Publication Year :
- 2019
-
Abstract
- Determination of ceftriaxone (CTRX) concentration in human cerebrospinal fluid (CSF) is required to clarify whether a high concentration of CTRX in CSF is associated with CTRX-induced encephalopathy (CIE). In our study, in order to perform an accurate analysis of CSF sample from CIE patient, we proposed HPLC with UV detection (HPLC-UV) using an octadecylsilica (ODS) column, a methanol and 10 mM phosphoric acid (25:75, v/v) mixture solution as a mobile phase, and a detection wavelength at 280 nm. The linear range was from 0.1 to 100 μg/mL (r = 0.999) in the present HPLC-UV. In the recovery tests using blank samples of human CSF and control serum spiked with CTRX, the recoveries of CTRX were >95.3%, and the RSD (n = 3) was <5.8%. We applied the proposed HPLC-UV system to determine CTRX in the CSF and serum samples obtained from a patient diagnosed as having CIE, and it was revealed that the CTRX concentrations in the CSF sample and the serum were 2.61 and 37.35 μg/mL, respectively. To the best of our knowledge, this is the first report describing the determination of CTRX concentration in a CSF sample obtained from a peritoneal dialysis patient diagnosed as having CIE. • An HPLC system was provided to determine ceftriaxone (CTRX) concentration. • Sample preparation for cerebrospinal fluid (CSF) analysis is simple. • The HPLC method showed accurate, precise, and selective CTRX determination. • A CSF sample from a peritoneal dialysis patient with CTRX-induced encephalopathy was analyzed. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15700232
- Volume :
- 1124
- Database :
- Academic Search Index
- Journal :
- Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 137418207
- Full Text :
- https://doi.org/10.1016/j.jchromb.2019.06.008