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Interferon-inducible lncRNA IRF1-AS represses esophageal squamous cell carcinoma by promoting interferon response.

Authors :
Huang, Jianbing
Li, Jiagen
Li, Yuan
Lu, Zhiliang
Che, Yun
Mao, Shuangshuang
Lei, Yuanyuan
Zang, Ruochuan
Zheng, Sufei
Liu, Chengming
Wang, Xinfeng
Li, Ning
Sun, Nan
He, Jie
Source :
Cancer Letters. Sep2019, Vol. 459, p86-99. 14p.
Publication Year :
2019

Abstract

Interferons (IFNs) play crucial roles in the development and treatment of cancer. Long non-coding RNAs (lncRNAs) are emerging molecules involved in cancer progression. Here, we identified and characterized an IFN-inducible nuclear lncRNA IRF1-AS (Interferon Regulatory Factor 1 Antisense RNA) which was positively correlated with IRF1 expression. IFNs upregulate IRF1-AS via the JAK-STAT pathway. Knockdown and overexpression of IRF1-AS revealed that IRF1-AS inhibits oesophageal squamous cell carcinoma (ESCC) proliferation and promotes apoptosis in vitro and in vivo. Mechanistically, IRF1-AS activates IRF1 (Interferon Regulatory Factor 1) transcription through interacting with ILF3 (Interleukin Enhancer Binding Factor 3) and DHX9 (DExH-Box Helicase 9). In turn, IRF1 binds to the IRF1-AS promoter directly and activates IRF1-AS transcription. Global analysis of IRF1-AS-regulated genes indicated that IRF1-AS activates the IFN response in vitro and in vivo. IRF1 knockdown in IRF1-AS-overexpressing cells abolished the antiproliferative effect and activation of the IFN response. Furthermore, IRF1-AS was downregulated in ESCC tissues, and low expression correlated with poor prognosis. In conclusion, the interferon-inducible lncRNA IRF1-AS represses esophageal squamous cell carcinoma progression by promoting interferon response through a positive regulatory loop with IRF1. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
03043835
Volume :
459
Database :
Academic Search Index
Journal :
Cancer Letters
Publication Type :
Academic Journal
Accession number :
137431536
Full Text :
https://doi.org/10.1016/j.canlet.2019.05.038