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Methylation status and expression patterns of myomaker gene play important roles in postnatal development in the Japanese flounder (Paralichthys olivaceus).
- Source :
-
General & Comparative Endocrinology . Sep2019, Vol. 280, p104-114. 11p. - Publication Year :
- 2019
-
Abstract
- • The methylation and expression of myomaker was assessed in Paralichthys olivaceus. • Myomaker expression increased throughout postnatal development. • Methylation was negatively correlated with myomaker expression. • Myomaker is likely a key myoblast fusion and muscle formation gene in P. olivaceus. Myomaker is a membrane protein that plays a crucial role in the fusion of myoblasts during muscle growth. DNA methylation, a significant factor, regulates gene expression. The aim of this study was to examine the methylation and mRNA expression patterns of the myomaker gene during 8 different postnatal developmental stages in the Japanese flounder (L: 7 days post hatch (dph); M1: 21 dph; M2: 28 dph; M3: 35 dph; J1: 90 dph; J2: 180 dph; A1: 24 months; A2: 36 months). Muscle tissue samples were taken from Japanese flounder at different postnatal development stages to measure the extent of DNA methylation and gene expression. Methylation level in the promoter and exon 1 of myomaker was measured using bisulfite sequencing, and the relative expression of myomaker during each developmental stage was measured by quantitative PCR. The relative expression levels of myomaker were up-regulated from stages L to M2, M3 to J2, and methylation of myomaker was negatively correlated with mRNA expression. Furthermore, the CpG site located at −26 bp in the promoter was the lowest methylated region in all developmental stages. These results offer a basis for understanding the mechanism by which myomaker regulates muscle formation during postnatal development. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PARALICHTHYS
*METHYLATION
*FLATFISHES
*DNA methylation
*MUSCLE growth
*P16 gene
Subjects
Details
- Language :
- English
- ISSN :
- 00166480
- Volume :
- 280
- Database :
- Academic Search Index
- Journal :
- General & Comparative Endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 137454499
- Full Text :
- https://doi.org/10.1016/j.ygcen.2019.04.017