Bioelectrocatalytic performance of d-fructose dehydrogenase.

This review summarizes the bioelectrocatalytic properties of d -fructose dehydrogenase (FDH), while taking into consideration its enzymatic characteristics. FDH is a membrane-bound flavohemo-protein with a molecular mass of 138 kDa, and it catalyzes the oxidation of d -fructose to 5-keto- d -fructose. The characteristic feature of FDH is its strong direct-electron-transfer (DET)-type bioelectrocatalytic activity. The pathway of the DET-type reaction is discussed. An overview of the application of FDH-based bioelectrocatalysis to biosensors and biofuel cells is also presented, and the benefits and problems associated with it are extensively discussed. Unlabelled Image • Bioelectrocatalytic properties of d -fructose dehydrogenase (FDH) are reviewed. • Recent findings about FDH are summarized especially DET mechanism. • The application of FDH to biosensors and biofuel cells are simmarized. • The benefits and problems of FDH-based reaction are detailed for future works. [ABSTRACT FROM AUTHOR]