Back to Search
Start Over
Transient kinetics of flavin-photosensitized oxidation of reduced redox proteins.
- Source :
-
European Journal of Biochemistry . 7/1/91, Vol. 199 Issue 1, p239-243. 5p. - Publication Year :
- 1991
-
Abstract
- We have used laser flash photolysis to investigate the kinetics of oxidation of reduced plastocyanins obtained from spinach and the green alga Monoraphidium braunii by the triplet states of lumiflavin, riboflavin and FMN. We have compared the results of these experiments with the kinetics of reduction of the oxidized forms of these proteins by the corresponding flavin semiquinones, as well as with the kinetics of flavin oxidation and reduction of cytochrome c552 (Class I c-type cytochrome, generic name c553) from Monoraphidium. in all cases, the rate constants for oxidation were one or two orders of magnitude larger than for reduction, consistent with the greater thermodynamic driving force for the oxidation reaction. Similar steric and electrostatic effects were observed for both reactions with all proteins, suggesting that the same (or closely adjacent) sites were being utilized for electron removal and entry. The two algal proteins were quite similar to one another in their redox properties, consistent with their physiological role of being able to substitute for one another in photosynthetic electron transport. In contrast, the algal plastocyanin was more reactive than the spinach protein in both oxidation and reduction, suggesting differences in their steric properties at the site of electron transfer. [ABSTRACT FROM AUTHOR]
- Subjects :
- *FLAVINS
*PIGMENTS
*OXIDATION-reduction reaction
*PROTEINS
*DYNAMICS
*BIOMOLECULES
Subjects
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 199
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 13747584
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1991.tb16115.x