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Cordycepin alleviates hepatic lipid accumulation by inducing protective autophagy via PKA/mTOR pathway.
- Source :
-
Biochemical & Biophysical Research Communications . Aug2019, Vol. 516 Issue 3, p632-638. 7p. - Publication Year :
- 2019
-
Abstract
- As the major active ingredient of Cordyceps militaris , cordycepin (3′-deoxyadenosine) has been well documented to possess lipid-lowering and anti-oxidative activities, making it a promising candidate for treatment of NAFLD. Autophagy was recently identified as a critical protective mechanism during NAFLD development. Therefore, this study aims to elucidate the mechanism of cordycepin regulating autophagy and lipid metabolism. Here, we found that cordycepin decreased palmitate-induced lipid accumulation by Oil Red O staining, Nile Red staining assays, triglyceride and total cholesterol measurements. Based on Western blot assay and immunocytochemistry, we found that cordycepin induced autophagy in PA-induced steatotic HepG2 cells. Whereas pretreatment with CQ, an autophagy inhibitor, substantially deteriorated the mitigative effects of cordycepin on PA-induced hepatic lipid accumulation. These data taken together indicate that cordycepin protects against PA-induced hepatic lipid accumulation via autophagy induction. Further, cordycepin remarkably increased the expression of P-PKA and decreased P-mTOR, whereas pretreatment with H89, a PKA inhibitor, abolished the ability of cordycepin to activate autophagy via mTOR activation. These data suggested that cordycepin protects against PA-induced hepatic lipid accumulation through the promotion of autophagy. The underlying mechanism might be associated with the PKA/mTOR pathway. Image 1 • Cordycepin decreased palmitate-induced lipid accumulation in HepG2 cells. • Cordycepin induced autophagy in PA-induced steatotic HepG2 cells. • Cordycepin decreased PA-induced hepatic lipid accumulation via autophagy induction. • The underlying mechanism might be associated with the PKA/mTOR pathway. [ABSTRACT FROM AUTHOR]
- Subjects :
- *LIPIDS
*LIPID metabolism
*OLEIC acid
*FATTY liver
*WESTERN immunoblotting
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 516
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 137591018
- Full Text :
- https://doi.org/10.1016/j.bbrc.2019.06.108