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Influence of Metal Ions on the Immobilization of β-Glucosidase Through Protein-Inorganic Hybrids.
- Source :
-
Indian Journal of Microbiology . Sep2019, Vol. 59 Issue 3, p370-374. 5p. - Publication Year :
- 2019
-
Abstract
- Immobilization of enzymes through metal-based system is demonstrated as a promising approach to enhance its properties. In this study, the influence of metals ions, including copper, cobalt and zinc (Zn) on the immobilization of β-glucosidase (BGL) through the synthesis of protein-inorganic hybrid was evaluated at 4 °C. Among these metal ions-based hybrids, Zn showed the highest encapsulation yield and relative activity of 87.5 and 207%, respectively. Immobilized BGL exhibited higher pH and temperature stability compared to free form. Thermal stability of hybrid improved up to 26-fold at 60 °C. After 10 cycles of reuse, immobilized enzyme retained 93.8% of residual activity. These results suggested that metal ions played a significant role in the enzyme immobilization as a protein-inorganic hybrid. Overall, this strategy can be potentially applied to enhance the properties of enzymes though effective encapsulation for the broad biotechnological applications. [ABSTRACT FROM AUTHOR]
- Subjects :
- *METAL ions
*COPPER ions
*THERMAL stability
*COBALT
*HIGH temperatures
Subjects
Details
- Language :
- English
- ISSN :
- 00468991
- Volume :
- 59
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Indian Journal of Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 137641860
- Full Text :
- https://doi.org/10.1007/s12088-019-00796-z