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Osteogenic potential of mesenchymal stem cells from rat mandible to regenerate critical sized calvarial defect.
- Source :
-
Journal of Tissue Engineering . Jan-Dec2019, Vol. 10, pN.PAG-N.PAG. 1p. - Publication Year :
- 2019
-
Abstract
- Although bone marrow–derived mesenchymal stem cells (MSCs) have been extensively explored in bone tissue engineering, only few studies using mesenchymal stem cells from mandible (M-MSCs) have been reported. However, mesenchymal stem cells from mandible have the potential to be as effective as femur-derived mesenchymal stem cells (F-MSCs) in regenerating bone, especially in the orofacial regions, which share embryonic origin, proximity, and accessibility. M-MSCs were isolated and characterized using mesenchymal stem cell–specific markers, colony forming assay, and multi-potential differentiation. In vitro osteogenic potential, including proliferation, osteogenic gene expression, alkaline phosphatase activity, and mineralization, was examined and compared. Furthermore, in vivo bone formations of F-MSCs and M-MSCs in rat critical sized defect were evaluated using microCT and histology. M-MSCs from rat could be successfully isolated and expanded while preserving their MSC's characteristics. M-MSCs demonstrated a comparable proliferation and mineralization potentials and in vivo bone formation as F-MSCs. M-MSCs is a promising cell source candidate for craniofacial bone tissue engineering. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 20417314
- Volume :
- 10
- Database :
- Academic Search Index
- Journal :
- Journal of Tissue Engineering
- Publication Type :
- Academic Journal
- Accession number :
- 137794297
- Full Text :
- https://doi.org/10.1177/2041731419830427