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Prevalence and antibiotic susceptibility of colistin-resistance gene (mcr-1) positive Enterobacteriaceae in stool specimens of patients attending a tertiary care hospital in Singapore.
- Source :
-
International Journal of Infectious Diseases . Aug2019, Vol. 85, p124-126. 3p. - Publication Year :
- 2019
-
Abstract
- • Unexpected high prevalence of mcr-1 in stool specimens of patients attending a tertiary care hospital. • Variable antibioltic-susceptibility profiles of mcr-1 positive Enterobacteriaceae isolates. • No detection of mcr-1 positive carbapenem-resistant Enterobacteriaceae. • First report of the prevalence of human faecal carriage of mcr- 1 in Singapore. The aim of this study was to determine the prevalence of the colistin-resistance gene (mcr-1) and the antibiotic-susceptibility profile of mcr-1 positive, colistin-resistant isolates in stool specimens of patients attending a tertiary care hospital in Singapore. 201 diarrheal stool specimens of patients attending the Changi General Hospital between May to August 2017 were collected and screened for the presence of mcr-1 by culture and molecular methods. Antibiotic-susceptibility profile of mcr -1 positive isolates was determined using the polymyxin B and colistin E-tests and the VITEK 2 system. We observed an unexpectedly high prevalence of mcr-1 in patients attending a tertiary care hospital in Singapore, i.e 6.0% and 8.0% estimated by stool culture and direct stool PCR, respectively. The mcr-1 gene was detected predominantly in Escherichia coli. Antibiotic-susceptibility testing on 12 mcr-1 positive Enterobacteriaceae isolates revealed variable susceptibility profiles with no detection of carbapenem-resistant Enterobacteriaceae. This is the first report of the prevalence of human faecal carriage of mcr- 1 in Singapore. Our findings highlight the potential risk of mcr- 1 spread among our patient cohort. The mcr-1 gene detection combined with the detection of other resistance gene targets of clinical importance is recommended to pre-empt the spread mcr-1 in our patients. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 12019712
- Volume :
- 85
- Database :
- Academic Search Index
- Journal :
- International Journal of Infectious Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 137825275
- Full Text :
- https://doi.org/10.1016/j.ijid.2019.05.029