Expression and serodiagnostic potential of antigen B and thioredoxin peroxidase from Taenia multiceps.

• Antigen B (AgB) and thioredoxin peroxidase (TPx) from Taenia multiceps were cloned. • The antigens were evaluated as agents for serological diagnosis of coenurosis. • Tm-AgB shows high sensitivity and thermal stability. • Tm-AgB is an excellent candidate antigen for coenurosis diagnosis kits. Coenurosis is a serious parasitic disease of herbivorous animals caused by the metacestode of Taenia multiceps (Coenurus cerebralis). Accordingly, a significant amount of research is currently dedicated to the development of appropriate antigens for use in rapid and accurate coenurosis diagnosis kits. In the present study, antigen B (AgB) and thioredoxin peroxidase (TPx) from T. multiceps were cloned and expressed using a prokaryotic system, molecular characterization of Tm -AgB was determined by bioinformatical analyses. The serological diagnostic potentials of r Tm -AgB and r Tm -TPx were evaluated by indirect ELISA and compared with those of previously reported r Tm -AnxB2, r Tm -HSP70, and r Tm -GST. The results showed that Tm -AgB is a specific lipoprotein of cestodes with good thermal stability. The ELISA assay showed that r Tm -AgB exhibited a sensitivity of 95.8% and a specificity of 87.5%, indicating its strong potential for serological diagnosis of T. multiceps. [ABSTRACT FROM AUTHOR]