基于cDNA-AFLP分析桂花开花进程中差异表达基因.

Osmanthus fragrans is an important traditional fragrant flower in China. Floral color and scent as the important ornamental characters are influenced by flowering process. Reports on the components of floral color and scent and related synthetic genes have been reported. However, studies on the synthesis and molecular basis of floral color and scent during flowering process are rarely reported. The study established a cDNA-AFLP system suitable for multiple samples and analyzed genes differential expression at different flowering stages. Finally, 283 TDFs with specific expression in petals and differential expression during flowering were successfully cloned and sequenced. Public databases blast showed that 120 TDFs had no homologous sequences, 12 TDFs had homologous sequences but their functions were unknown, and 150 TDFs had known biological functions, including the secondary metabolism, primary metabolism and development process, ect. qRT-PCR results of 6 TDFs with known functions comparing with cDNA-AFLP displayed that 4 TDFs with higher transcriptional level had consistent expression level. These results would be helpful to understand the gene expression of petals during flowering processes, and provide useful information for exploiting the mechanism of the floral color and scent formation of O. fragrans from molecular level. [ABSTRACT FROM AUTHOR]