深层海水促进糖尿病模型小鼠创面愈合：激活Wnt/β-catenin通路的 机制研究.

BACKGROUND: Deep seawater has been shown to effectively alleviate the process of diabetes in mice induced by high fat diet through activating AMPK pathway, but there are few studies about the role of deep seawater in the wound healing of diabetes mellitus. OBJECTIVE: To explore the mechanism of deep seawater on the cutaneous wound healing in diabetic mice. METHODS: Forty-eight healthy male Kunming mice (provide by Laboratory Animal Center of Kunming Medical University) were selected and were given intraperitoneal injection of streptozotocin to establish diabetic model. After 14 days of incubation, the back would model was established, and the mouse models were randomly divided into four groups: control group (purified water), tap water group, deep seawater group and inhibitor group (deep seawater + venous injection of Wnt/β-catenin inhibitor, XAV-939). Subsequently, the blood glucose and body mass of model mice were observed at 3, 7 and 10 days after wound modeling, and the healing of the wound was observed. The morphological changes of the wound were evaluated. The expression levels of β-catenin, GSK-3β and Rspo-3, malondialdehyde, superoxide dismutase and glutathione peroxidase in the serum were detected. The study was approved by the Laboratory Animal Ethics Committee of Kunming Institute of Zoology, China Academy of Sciences, approval No. KPRC-2017091. RESULTS AND CONCLUSION: (1) Deep seawater showed no significant effect on the body mass and blood glucose. (2) The wound healing rate in the deep seawater group was significantly higher than that in the tap water and control groups at different time points (all P < 0.01). (3) Western blot results showed that the expression levels of β-catenin and Rspo-3 were higher in the deep seawater group than the control and tap water groups, but GSK-3β was down-regulated. (4) Hematoxylin-eosin staining showed that compared with the control group, vascular endothelial cells, new capillaries and fibroblast cells proliferation in the deep seawater group were increased, but the number of inflammatory cells got opposite results. However, there was no significant difference between the deep seawater + XAV-939 group and control group (P > 0.05). The healing rate of wound at different time points in the deep seawater group was significantly higher than that in the tap water and control groups (all P < 0.01). (5) Compared with the control group, the expression levels of superoxide dismutase and glutathione peroxidase in serum in the deep seawater group were increased (P < 0.01 and P < 0.001), and malondialdehyde decreased (P < 0.05 and P < 0.01). There was no significant difference between deep seawater + XAV-939 and control groups (P > 0.05). (6) To conclude, deep seawater promotes cutaneous wound healing in diabetic mice via activating Wnt/β-catenin pathway. [ABSTRACT FROM AUTHOR]