Detection of Theileriaannulata isolated from cattle using Real-Time PCR.

This study aimed to molecular investigation the prevalence of Theileriaannulataparasite that isolated from blood samples of cattle. A hundred of blood sampleswascollected randomanly from cattles ofthe middle area of Iraq (Babylon province). Samples were submitted to blood DNA extraction assay. Nano drop spectrophotometer and QRT-PCR with TaqMan methods were involved for the surface antigen Tams1detection of Theileriaannulata. Real- Time PCR results showed only 16 samples were positive out of 50 blood samples with a percentage 32%. The threshold cycle (CT value) of these positive samples were ranged from CT: 24.29 to CT: 35.69. The Real-Time PCRefficiency was validated using standard curve method, and the efficiently was approximately (99.9%).Surprisingly, the overall results from current study showed there were no association between ages, gender. Infectionsdistribution between (1-12month) (28.57%), 1-2 year (31.57%) and 3-10 year (29.4%).Additionally, the ratio of the infected females at age of 1-2yearswere twice more than the ratio of infected males. [ABSTRACT FROM AUTHOR]