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Expression and characterization of cholera toxin B—pneumococcal surface adhesin A fusion protein in Escherichia coli: ability of CTB-PsaA to induce humoral immune response in mice
- Source :
-
Biochemical & Biophysical Research Communications . Aug2004, Vol. 321 Issue 1, p192-196. 5p. - Publication Year :
- 2004
-
Abstract
- Cholera toxin B subunit (CTB) is responsible for CT holotoxin binding to the cell and has been described as a mucosal adjuvant for vaccines. In this work, the ctxB gene was genetically fused to the psaA gene from Streptococcus pneumoniae, a surface protein involved in its colonization in the host that is also considered a vaccine antigen candidate against this pathogen. The CTB-PsaA fusion protein was expressed in Escherichia coli, and the purified protein was used for intranasal immunization experiments in Balb/C mice. CTB-PsaA was able to induce both systemic and mucosal antibodies evaluated in serum, saliva, and in nasal and bronchial wash samples, showing that CTB-PsaA is a promising molecule to be investigated as S. pneumoniae vaccine antigen candidate. [Copyright &y& Elsevier]
- Subjects :
- *PREVENTIVE medicine
*CHOLERA toxin
*ESCHERICHIA coli
*TOXINS
*BIOLOGICALS
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 321
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 13808093
- Full Text :
- https://doi.org/10.1016/j.bbrc.2004.06.118