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Autoinducer-2 of gut microbiota, a potential novel marker for human colorectal cancer, is associated with the activation of TNFSF9 signaling in macrophages.
- Source :
-
OncoImmunology . 2019, Vol. 8 Issue 10, p1-8. 8p. - Publication Year :
- 2019
-
Abstract
- Objectives: The interaction between the quorum sensing (QS) molecules of gut microbiota and the immunity of colorectal cancer (CRC) has not been investigated before. Methods: We measured the concentration of autoinducer-2 (AI-2) in samples of stool, colorectal tissue, saliva and serum of CRC patients, and compared this to AI-2 levels in colorectal adenoma (AD) and normal colon mucosa (NC). To explore the activated signaling pathways involved, we utilized AI-2 extracted from Fusobacterium nucleatum to stimulate macrophages and validated these in vitro findings in human CRC tissues. Results: The AI-2 concentration in both colorectal tissue and stool of CRC patients was significantly higher when compared to that in AD and NC (all P values <.01). The AI-2 concentration along with the progression of CRC in both tissues and stools was significantly increased (P=.045,P=.0003, respectively). After AI-2 stimulation, TNFSF9 was the most significantly increased protein in macrophage cells (P <.01). TNFSF9 expression was significantly higher in CRC tissues when compared to NCs (P<.0001), which was mainly derived from macrophages in the tumor microenvironment. Moreover, AI-2 level was positively associated with CD3 + T cell numbers (P=.0462), and negatively associated with CD4/CD8 ratio (P=.0113) within CRC tissues. Conclusions: We demonstrated for the first time that AI-2 may serve as a novel marker for screening CRC in the clinic. AI-2 was associated with tumor immunity in CRCs through tumor-associated macrophages and CD4/CD8 ratio in a TNFSF9-dependent manner. [ABSTRACT FROM AUTHOR]
- Subjects :
- *GUT microbiome
*COLORECTAL cancer
*QUORUM sensing
*TUMOR microenvironment
*T cells
Subjects
Details
- Language :
- English
- ISSN :
- 21624011
- Volume :
- 8
- Issue :
- 10
- Database :
- Academic Search Index
- Journal :
- OncoImmunology
- Publication Type :
- Academic Journal
- Accession number :
- 138173040
- Full Text :
- https://doi.org/10.1080/2162402X.2019.1626192