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Physicochemical characterization and antioxidant activity of cell-bound exopolysaccharides from Lactobacillus fermentum S1 obtained by two extraction methods.
- Source :
-
Process Biochemistry . Oct2019, Vol. 85, p195-205. 11p. - Publication Year :
- 2019
-
Abstract
- • Two novel cell-bound exopolysaccharides (c-EPS) were extracted using phenol and EDTA. • Extraction method significantly affects structure and antioxidant activity of c-EPS. • Two types of c-EPS presented different surface morphologies and thermal properties. • Both c-EPS exhibited potent antioxidant properties in vitro and in vivo. This study investigated the physicochemical characteristics and antioxidant activities of cell-bound exopolysaccharides (c-EPS) from Lactobacillus fermentum S1 extracted using either phenol (CFP) or ethylenediaminetetraacetic acid (CFE). The extraction products, CFP and CFE, were homogeneous polysaccharides with molecular weights of 2.89 × 105 and 2.44 × 105 Da, respectively. Both CFP and CFE were composed of five kinds of monosaccharides. They had a main chain of β-D-Glc p -(1→2), α-L-Rha p -(1→2), β-D-Gal p -(1→3), α-D-Glc p- (1→3), α-D-Gal p -(1→3) and β-L-Rha p- (1→3,4) residues, and a tail of α-D-Man p -(1→) residue, but with different molar ratios. Trace amounts of D-Gal p- (1→) and L-Ara f -(1→2,5) residues were randomly distributed in the sugar chains. Although both CFP and CFE were in triple-helical conformation, they differed from each other in relation to surface morphology and thermal properties. The two c-EPS had different performances in radical scavenging and ferric reducing antioxidant power assays in vitro. The antioxidant capacity of both c-EPS extracts was confirmed in vivo using Caenorhabditis elegans , whose total antioxidant capacity and total superoxide dismutase activity increased after c-EPS treatment. Overall, the extraction method significantly affected the structure and biological activity of c-EPS. Moreover, the potent antioxidant activities of CFP and CFE support their use as natural antioxidants in dietary supplements or food additives. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13595113
- Volume :
- 85
- Database :
- Academic Search Index
- Journal :
- Process Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 138292826
- Full Text :
- https://doi.org/10.1016/j.procbio.2019.06.017