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On-chip immuno-agglutination assay based on a dynamic magnetic bead clump and a sheath-less flow cytometry.
- Source :
-
Biomicrofluidics . 2019, Vol. 13 Issue 4, pN.PAG-N.PAG. 10p. - Publication Year :
- 2019
-
Abstract
- Immunoagglutination assay is a promising approach for the detection of waterborne analytes like virus, cells, proteins with its advantages such as a smaller amount of reagents and easier operation. This paper presents a microfluidic agglutination assay on which all the assay processes including analyte capture, agglutination, and detection are performed. The chip integrates an on-chip pump for sample loading, a dynamic magnetic bead (MB) clump for analyte capture and agglutination, and a sheath-less flow cytometry for particle detection, sizing, and counting. The chip is tested with streptavidin-coated MBs and biotinylated bovine serum albumin as a model assay, which realizes a limit of detection (LOD) of 1 pM. Then, an antigen/antibody assay using rabbit IgG and goat anti-rabbit IgG coated MBs is tested and a LOD of 5.5 pM is achieved. At last, human ferritin in 10% fetal bovine serum is tested with Ab-functionalized MBs and the detection achieves a LOD of 8.5 pM. The whole procedure takes only 10 min in total. [ABSTRACT FROM AUTHOR]
- Subjects :
- *FLOW cytometry
*STREPTAVIDIN
*FERRITIN
*SERUM albumin
*GRANULAR flow
*BEADWORK
Subjects
Details
- Language :
- English
- ISSN :
- 19321058
- Volume :
- 13
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Biomicrofluidics
- Publication Type :
- Academic Journal
- Accession number :
- 138400700
- Full Text :
- https://doi.org/10.1063/1.5093766