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In vitro shoot multiplication of Rumex vesicarius L., and quantification of ascorbic acid and major phenolics from its leaf derived callus.
- Source :
-
3 Biotech . 9/4/2019, Vol. 9 Issue 10, pN.PAG-N.PAG. 1p. - Publication Year :
- 2019
-
Abstract
- An optimised method for enhanced in vitro shoot multiplication of Rumex vesicarius (Polygonaceae)—a branched succulent herb—was achieved. The in vitro seed pre-treatment with 4% urea was able to show 95% seed germination on MS medium within 2 weeks of culturing. In vitro shoot bud induction from shoot tip explants was best in the presence of 2 mg L−1 kinetin on Murashige and Skoog medium (MS medium), wherein 8.3 shoots/explants were observed. Shoot elongation was found to be high (3.75 ± 0.5 cm) in 2 mg L−1 BA comprising medium. The transfer of micro-shoots on to MS medium comprising 1.5 mg L−1 IBA and 1% activated charcoal (w/v) supported efficiently in vitro rooting (2.5–4.0 cm) in 3–4 weeks. Upon hardening, 70% rooted plants survived under greenhouse conditions. Though friable callus was produced on MS medium-containing 2 mg L−1 BA followed by 3 mg L−1 BA, no organogenesis was noticed. The ascorbic acid content of 78.62 ± 0.25 mg 100 g−1 FW was recorded in callus cultures grown on medium supplemented with 2 mg L−1 BA, and it is 1.74-fold more compared to normal ex vitro leaves of the same age. In vitro raised plant leaf showed 1.98-fold more ascorbic acid (89.42 ± 0.18 mg 100 g−1 FW) to that of ex vitro leaves. The total phenolic content was found to be 60 mg in callus as compared to 610 mg (per 100 g GAE FW) of ex vitro leaves. The major phenolic compounds quantified were synergic, chlorogenic, ferulic, and generic acids, respectively. This optimised protocol will facilitate to pursue scale-up studies for in vitro ascorbic acid production and also to further investigate the kinetics of biosynthetic pathway genes involved. [ABSTRACT FROM AUTHOR]
- Subjects :
- *VITAMIN C
*CALLUS
*RUMEX
*ACTIVATED carbon
*GERMINATION
*PLANT shoots
Subjects
Details
- Language :
- English
- ISSN :
- 2190572X
- Volume :
- 9
- Issue :
- 10
- Database :
- Academic Search Index
- Journal :
- 3 Biotech
- Publication Type :
- Academic Journal
- Accession number :
- 138431230
- Full Text :
- https://doi.org/10.1007/s13205-019-1877-3