Structural changes of soil organic matter and the linkage to rhizosphere bacterial communities with biochar amendment in manure fertilized soils.

The molecular structure of soil organic matter (SOM) is closely related to the structure and functions of the rhizosphere community. In this study, a microcosm experiment was conducted to investigate the effect of straw-derived biochar on the structural differentiation of SOM and rhizosphere bacterial communities. 13C NMR spectra showed that the relative abundance of carboxyl, alkyl, N-alkyl, O-alkyl and methoxyl groups decreased by 22%, 31%, 27%, 27% and 27%, respectively, and the relatively stable structure of aryl and O-aryl groups increased by 213% and 41%, respectively. In addition, network-based analysis showed that biochar amendment not only improved the network connectivity of the rhizosphere bacteria, but also enhanced the linkage between bacteria and the molecular structure of SOM. The topological properties of the network, including the number of edges, network density, and average clustering coefficient, were increased by 79%, 15% and 22%, respectively. Moreover, modularization analysis and structural equation model indicated that rhizosphere bacterial network was the important bio-factor affecting the content of labile carbon. Unlabelled Image • Biochar amendment strengthened network connectivity among rhizosphere bacteria. • The linkage between soil carbon and rhizosphere bacterial communities was enhanced by biochar. • Change of rhizosphere bacterial network was the important bio-factor affecting molecular structure of SOM. [ABSTRACT FROM AUTHOR]