Catalytic hairpin assembly induced dual signal enhancement for rapid detection of miRNA using fluorescence light-up silver nanocluster.

A novel method of catalytic hairpin assembly (CHA) induced dual signal enhancement is developed for rapid detection of miRNA based on fluorescence light-up silver nanocluster (Ag NC). By the hybridization of a hairpin DNA and a single-stranded DNA, a unique probe is firstly designed. In the terminals of this probe, DNA-Ag NCs can be formed and display very weak fluorescence. In the presence of the target miRNA, the reaction of CHA can be triggered, forming two kinds of double-stranded complexes, in which the terminal DNA-Ag NCs are in close proximity to G-rich overhangs and the fluorescent signal can be dramatically enhanced. Compared with many other enzyme-based amplification strategies, this one exhibits distinct advantages of simplicity in experimental operation and a rapid detection process (within 1 h). Moreover, this assay exhibits an excellent selectivity and is successfully applied in the detection of miRNAs in complex biological media, which confirms the reliability and practicality of this protocol. A novel method of catalytic hairpin assembly (CHA) induced dual signal enhancement is developed, which has been used for rapid detection of miRNA based on fluorescence light-up silver nanocluster. Image 1 • A novel method of catalytic hairpin assembly induced dual signal enhancement is developed. • The method is used for miRNA detection based on fluorescence light-up silver nanocluster. • This assay has the advantages of simplicity in experimental operation and a rapid detection process. [ABSTRACT FROM AUTHOR]