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Oral delivery of single-chain insulin (SCI-59) analog by bacterium-like particles (BLPs) induces oral tolerance and prevents autoimmune diabetes in NOD mice.
- Source :
-
Immunology Letters . Oct2019, Vol. 214, p37-44. 8p. - Publication Year :
- 2019
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Abstract
- • Single chain insulin (SCI) is delivered by bacteria bacterium like particles (BLPs). • Oral vaccination with BLPs-SCI-59 efficiently induces. antigen-specific tolerance. • Oral vaccination with BLPs-SCI-59 efficiently prevents diabetes in NOD mice. • BLPs may be used as an antigen delivery vehicle to induce immune tolerance. Oral tolerance, induced by oral administration of autoantigens, is a promising therapeutic approach to treat type 1 diabetes mellitus (T1DM). However, the degradation of antigens passing through the gastrointestinal tract (GIT) leads to low induction efficiency. Based on our previous study, a single-chain insulin (SCI-59) analog, bound to the surface of lactic acid bacteria (LAB) bacterium-like particles (BLPs), was more stable in the simulated gastric fluid, compared to free SCI-59 and insulin. Based on the analysis of diabetes progression, a significant decrease in the incidence of diabetes was observed in mice fed BLPs-SCI-59. Oral administration of BLPs-SCI-59 can enhance glucose tolerance in NOD mice and this effect may result from the protection of pancreatic islet beta cells, as compared to the free SCI-59 group and BLPs group. Oral administration of BLPs-SCI-59 can significantly reduce insulitis and preserve the ability of insulin secretion in treated mice. Oral vaccination with BLPs-SCI-59 induced SCI-59 specific T cell tolerance in treated mice, which may due to the repair of Th1/Th2 imbalance and increased CD4+CD25+FoxP3+ regulatory T cells (Tregs). These results show that oral vaccination with BLPs-SCI-59 is a promising way to prevent T1DM in NOD mice. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01652478
- Volume :
- 214
- Database :
- Academic Search Index
- Journal :
- Immunology Letters
- Publication Type :
- Academic Journal
- Accession number :
- 138668048
- Full Text :
- https://doi.org/10.1016/j.imlet.2019.08.008