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A FRET-based ratiometric fluorescent probe for highly selective detection of cysteine based on a coumarin–rhodol derivative.
- Source :
-
New Journal of Chemistry . 10/7/2019, Vol. 43 Issue 37, p14763-14771. 9p. - Publication Year :
- 2019
-
Abstract
- Cysteine, as an important amino acid in the human body, plays a vital role in people's normal life activities. In this paper, a ratiometric fluorescent probe for detecting cysteine was designed and synthesized based on the fluorescence resonance energy transfer (FRET) process. In this FRET system, a coumarin derivative was used as the energy donor, a rhodol fluorophore was chosen as the energy receptor, and an acrylate group was utilized as a cysteine recognition unit. In the absence of cysteine, the rhodol receptor was in the non-fluorescent lactone state and the FRET process was inhibited. Upon addition of cysteine, the closed spirolactone form was converted to a conjugated fluorescent xanthene form to induce the occurrence of FRET which resulted in a fluorescent signal decrease at 470 nm and enhancement at 543 nm. The ratiometric fluorescent probe exhibited excellent selectivity to Cys over Hcy and GSH. In addition, I543nm/I470nm of the probe for cysteine displayed a good linear relationship in the range of 5.0 × 10−7–1.0 × 10−4 mol L−1, and the detection limit was 2.0 × 10−7 mol L−1. Furthermore, the probe showed low cell toxicity and was successfully applied to the confocal imaging of cysteine in HepG2 cells using dual emission channels. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CYSTEINE
*FLUORESCENT probes
*FLUORESCENCE resonance energy transfer
*COUMARINS
Subjects
Details
- Language :
- English
- ISSN :
- 11440546
- Volume :
- 43
- Issue :
- 37
- Database :
- Academic Search Index
- Journal :
- New Journal of Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 138777782
- Full Text :
- https://doi.org/10.1039/c9nj03375k