破骨细胞因子对体外培养胎鼠DRG内疼痛相关蛋白表达的影响.

Objective To investigate the effects of osteoclast factors on pain-related protein expression in fetal dorsal root ganglion (DRG) cultured in vitro. Methods We raised 20 embryos to the 15tli day of pregnancy to perform embryo retrieval, and we extracted fetal rat DRG for in vitro culture for 48 hours and then they were randomly divided into 6 groups; the control group (containing 0.1% PBS), transforming growth factor (2, (TGF-β1 treatment group (containing 10 ng/mL TGF-β1 ) , insulin-like growth factor II (IGF-II ) treatment group (containing 1 μ/mL IGF-II ) , basic fibroblast growth factor (bFGF) treatment group (containing 10 ng/mL bFGF), platelet-derived growth factor (PDGF) AA treatment group ( containing 50 ng/mL PDGF-AA), and bone morphogenic protein 5 (BMP-5) treatment group (containing 10 μ/mL BMP-5). Western blotting and real-time fluorescence quantitative PGR were applied to analyze the protein and mRNA expression of galanin (Gal) , Gal receptor 1 ( GalRl ) , Gal receptor 2 ( GalR2 ), substance P (SP) , calcitonin gene-related peptide (CGRP). Results Compared with the control group, the protein expression of CGRP, Gal, and SP in the other groups was up-regulated (all P<0.05) , the GalRl protein expression in TGF-β1, group and PDGF-AA group was down-regulated (P<0.05), and the GalR2 protein expression in TGF-β1, group, bFGF group, and PDGF- A group was up-regulated (P<0.05). Compared with the control group, the relative mRNA expression levels of CGRP and Gal in the other treatment groups increased (all P<0.05) , the mRNA expression level of GalRl in the TGF-β1, group and PDGF-AA group decreased (P<0.05), the mRNA expression level of GalR2 in TGF-β1, group, bFGF group, and PDGF-AA group increased (P<0.05), and the mRNA expression level of SP in TGF-β1, group, IGF-II group, bFGF group, and PDGF-AA group increased (P<0.05). Conclusion Osteoclasts factors TGF-β1, , IGF-II , bFGF, PDGF-AA, and BMP-5 have an effect on the expression of pain-related proteins Gal, GalRl , GalR2, SP, and CGRP in DRG in vitro. [ABSTRACT FROM AUTHOR]