Somatic antigen of <em>Pseudomonas aeruginosa</em>.

The lipopolysaccharides from two serologically related strains of Pseudomonas aeruginosa II (Sandvik classification) and V (IM-1, Verder-Evans classification) were isolated by the Westphal method and cleaved with 1% acetic acid. The O-specific polysaccharide from Sandvik II lipopolysaccharide involved L-rhamnose, N-acetyl-D-quinovosamine, and N-acetyl-D-galactosaminuronic acid in the ratio 2:1:1, as well as O-acetyl groups. The Verder-Evans V O-specific polysaccharide was found to contain just the same sugars and, in addition, D-glucose, the monosaccharide ratio being 2:1:1:1. On the basis of methylation analysis, Smith degradation, solvolysis with anhydrous hydrogen fluoride and ¹H and 13C nuclear magnetic resonance data, it was concluded that the polysaccharides have identical main chains, and there were established the following structures of their repeating units: ... The data obtained allow us to substantiate the combining of the strains studied into one O-serogroup as two individual O-serotypes. [ABSTRACT FROM AUTHOR]