高原鼠兔与大鼠低氧后外周血及骨髓红系细胞变化特点.

BACKGROUND: Changes of red blood cell in peripheral blood and bone marrow erythropoietic system in plateau pikas are of great significance for hypoxic adaptation. OBJECTIVE: To explore the hypoxic adaptation of erythropoietic system in plateau pikas by comparing the morphological changes of peripheral blood and bone marrow between plateau pikas and rats exposed to hypoxia. METHODS: There were 12 healthy wild plateau pikas and 12 clean Sprague-Dawley rats and were randomly divided into experimental and control groups, 6 in each group. The experimental group animals were fed in a simulated 5 000 m altitude hypobaric hypoxia chamber for 28 consecutive days, and the control group animals were fed in the laboratory at 2 260 m altitude. RESULTS AND CONCLUSION: (1) The diameter of the erythrocytes was smaller and red blood cell count was higher in plateau pikas than those in the rats of control group. After 28 days of hypoxia, red blood cell count, hemoglobin and hematocrit were increased in both experimental groups (P < 0.001), but the increased rate of plateau pikas were less than that of the rats. The mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration showed no significant changes in plateau pikas. (2) Results of bone marrow smear showed that the proportion of polychromatic and orthochromatic erythroblasts had no significant changes in plateau pikas after hypoxia, but increased significantly in the rats (P < 0.05). (3) Hematoxylin-eosin staining results of the sternum indicated that the immature erythroblasts islands did not change significantly in plateau pikas, but increased significantly in rats. (4) So the erythroid changes in peripheral blood and bone marrow of plateau pikas before and after hypoxia are significantly lower than those of the Sprague-Dawley rats, and they may be related to the hypoxia adaptation mechanism. [ABSTRACT FROM AUTHOR]