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The origin of amniotic fluid monocytes/macrophages in women with intra-amniotic inflammation or infection.
- Source :
-
Journal of Perinatal Medicine . Oct2019, Vol. 47 Issue 8, p822-840. 19p. 2 Diagrams, 1 Chart, 3 Graphs. - Publication Year :
- 2019
-
Abstract
- Background: Monocytes, after neutrophils, are the most abundant white blood cells found in the amniotic cavity of women with intra-amniotic inflammation/infection. However, the origin of such cells has not been fully investigated. Herein, we determined (1) the origin of amniotic fluid monocytes/macrophages from women with intra-amniotic inflammation/infection, (2) the relationship between the origin of amniotic fluid monocytes/macrophages and preterm or term delivery and (3) the localization of monocytes/macrophages in the placental tissues. Methods: Amniotic fluid samples (n = 16) were collected from women with suspected intra-amniotic inflammation or infection. Amniotic fluid monocytes/macrophages were purified by fluorescence-activated cell sorting, and DNA fingerprinting was performed. Blinded placental histopathological evaluations were conducted. Immunohistochemistry was performed to detect CD14+ monocytes/macrophages in the placental tissues. Results: DNA fingerprinting revealed that (1) 56.25% (9/16) of amniotic fluid samples had mostly fetal monocytes/macrophages, (2) 37.5% (6/16) had predominantly maternal monocytes/macrophages and (3) one sample (6.25% [1/16]) had a mixture of fetal and maternal monocytes/macrophages. (4) Most samples with predominantly fetal monocytes/macrophages were from women who delivered early preterm neonates (77.8% [7/9]), whereas all samples with mostly maternal monocytes/macrophages or a mixture of both were from women who delivered term or late preterm neonates (100% [7/7]). (5) Most of the women included in this study presented acute maternal and fetal inflammatory responses in the placenta (85.7% [12/14]). (6) Women who had mostly fetal monocytes/macrophages in amniotic fluid had abundant CD14+ cells in the umbilical cord and chorionic plate, whereas women with mostly maternal amniotic fluid monocytes/macrophages had abundant CD14+ cells in the chorioamniotic membranes. Conclusion: Amniotic fluid monocytes/macrophages can be of either fetal or maternal origin, or a mixture of both, in women with intra-amniotic inflammation or infection. These immune cells could be derived from the fetal and maternal vasculature of the placenta. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ANTIGEN analysis
*BACTERIAL disease complications
*DIAGNOSIS of bacterial diseases
*DIAGNOSIS of fetal diseases
*AMNIOTIC liquid
*DNA fingerprinting
*FETAL diseases
*IMMUNOHISTOCHEMISTRY
*INFANT health services
*PREMATURE infants
*PREMATURE labor
*MACROPHAGES
*MONOCYTES
*PLACENTA
*PREGNANT women
*RISK assessment
*DISEASE complications
*FETUS
Subjects
Details
- Language :
- English
- ISSN :
- 03005577
- Volume :
- 47
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- Journal of Perinatal Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 139036306
- Full Text :
- https://doi.org/10.1515/jpm-2019-0262