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Somatic antigens of <em>Pseudomonas aeruginosa</em>.
- Source :
-
European Journal of Biochemistry . 3/16/87, Vol. 163 Issue 3, p627-637. 11p. 2 Diagrams, 2 Charts. - Publication Year :
- 1987
-
Abstract
- The O-specific polysaccharide, obtained on mild acid degradation of lipopolysaccharide of <em>Pseudomonas aeruginosa</em> O13 (Lányi classification), is built up of trisaccharide repeating units involving 2-acetamidino-2,6-dideoxy-D-glucose (N-acetyl-D-quinovosamine, D-QuiNAc), 2-acetamidino-2,6-dideoxy-L-galactose (L- fucosacetamidine, L-FucAm), and a new sialic-acid-like sugar, 5,7-diacetamido-3,5,7,9-tetradeoxy- D-<em>glycero-L-galacto</em>-nonulosonic acid (Sug), and thus contains simultaneously both acidic and basic functions. Cleavage of the polysaccharide with hydrogen fluoride in methanol revealed the high stability of the glycosidic linkage of the ulosonic acid and afforded methyl glycosides of a disaccharide and a trisaccharide. The structures of the new ulosonic acid and acetamidino group were established by analysing the oligosaccharide fragments by 1H, 13C nuclear magnetic resonance spectrometry, as well as on the basis of their chemical conversions: alkaline hydrolysis of the acetamidino group into acetamido group, reductive deamination with lithium borohydride into the ethylamino group and acetylation with acetic anhydride in pyridine accompanied by intramolecular acylation of the acetamidino function by the ulosonic acid to form a six-membered lactam ring. Identification of the oligosaccharide fragments and comparative analysis of the 13C nuclear magnetic resonance spectra of the oligosaccharides and polysaccharide revealed the following structure of the repeating unit:... [ABSTRACT FROM AUTHOR]
- Subjects :
- *ANTIGENS
*PSEUDOMONAS aeruginosa
*POLYSACCHARIDES
*ENDOTOXINS
*HYDROFLUORIC acid
Subjects
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 163
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 13919225
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1987.tb10912.x