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Two c-type lectins from Venerupis philippinarum: Possible roles in immune recognition and opsonization.
- Source :
-
Fish & Shellfish Immunology . Nov2019, Vol. 94, p230-238. 9p. - Publication Year :
- 2019
-
Abstract
- In the study, two c-type lectins were identified and characterized from the manila clam Venerupis philippinarum (designed as VpClec-1 and VpClec-2, respectively). Multiple alignments and phylogenetic analysis strongly suggested that they were new members of the c-type lectin superfamily. In normal tissue of clams, both VpClec-1 and VpClec-2 transcripts were highly expressed in the tissue of hepatopancreas. After Vibrio anguillarum challenge, the temporal expression of both VpClec-1 and VpClec-2 transcripts was up-regulated in the hemocytes of manila clams. The recombinant protein VpClec-1 (rVpClec-1) showed obvious binding activities to lipopolysaccharide (LPS), peptidoglycan (PGN), glucan and zymosan in vitro , while the recombinant protein VpClec-2 (rVpClec-2) could only bind LPS, glucan and zymosan. Coinciding with the PAMPs binding assay, both rVpClec-1 and rVpClec-2 displayed broad agglutination and antibacterial activities towards Vibrio harveyi , Vibrio splendidus , Vibrio anguillarum , Enterobacter cloacae and Aeromonas hydrophila. Moreover, the phagocytosis and encapsulation ability of hemocytes could be significantly enhanced by rVpClec-1 and rVpClec-2. Notably, the rVpClec-1 but not rVpClec-2 elicited a chemotactic response from hemocytes. All the results showed that VpClec-1 and VpClec-2 functioned as pattern recognition receptors (PRRs) with distinct recognition spectrum, and involved in the innate immune responses of manila clams. • Two c-type lectins (VpClec-1 and VpClec-2) were identified from the Manila clam Ruditapes philippinarum. • Both VpClec-1 and VpClec-2 were highly expressed in hepatopancreas of healthy Manila clam. • Both VpClec-1 and VpClec-2 showed a broad spectrum of microbial binding and opsonic activities. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10504648
- Volume :
- 94
- Database :
- Academic Search Index
- Journal :
- Fish & Shellfish Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 139218021
- Full Text :
- https://doi.org/10.1016/j.fsi.2019.09.009