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Ultrasound pretreatment enhances the inhibitory effects of nisin/carvacrol against germination, outgrowth and vegetative growth of spores of Bacillus subtilis ATCC6633 in laboratory medium and milk: Population and single-cell analysis.
- Source :
-
International Journal of Food Microbiology . Dec2019, Vol. 311, pN.PAG-N.PAG. 1p. - Publication Year :
- 2019
-
Abstract
- This study evaluated the synergetic inhibitory effects of ultrasound and nisin/carvacrol on spore germination, outgrowth, and subsequent growth of vegetative cell of Bacillus subtilis in laboratory medium and milk. Ultrasound pretreatment (3.33 W/mL, 15 min) and nisin/carvacrol (0.01%, 0.02%) synergistically inhibited spore germination, outgrowth, and vegetative growth of spores in laboratory medium. Whereas no such inhibitory effect was observed in milk even with a 10-fold increase in the concentration (1%) of nisin. Flow cytometry analysis showed that the germination capacities of ultrasound pretreated spores combined with nisin/carvacrol (67.3% and 30.5%, respectively) was lower than that of the untreated spores (95.1%). These results quantitatively revealed the inhibitory effect of the combined treatments which were confirmed by phase-bright spore observations at single cell level. In general, the current work identified the combined ultrasound-carvacrol treatment as an effective strategy to control spores and vegetative cells of B. subtilis in the laboratory medium and milk during abusive storage. • Synergism of ultrasound and nisin/carvacrol demonstrated in laboratory medium. • Spores stressed with the combined treatments were more heterogeneously. • The combined treatments could control B. subtilis in milk better than individual treatment. [ABSTRACT FROM AUTHOR]
- Subjects :
- *BACILLUS subtilis
*SPORES
*GERMINATION
*MILK
*CARVACROL
Subjects
Details
- Language :
- English
- ISSN :
- 01681605
- Volume :
- 311
- Database :
- Academic Search Index
- Journal :
- International Journal of Food Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 139386797
- Full Text :
- https://doi.org/10.1016/j.ijfoodmicro.2019.108329