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Development of an immunochromatography assay for salinomycin and methyl salinomycin in honey.
- Source :
-
Food & Agricultural Immunology . Dec2019, Vol. 30 Issue 1, p995-1006. 12p. - Publication Year :
- 2019
-
Abstract
- This study developed a sensitive monoclonal antibody, 3A3, to salinomycin (SLM) and methyl salinomycin (MLN). The monoclonal antibody 3A3 is highly specific for SLM with an antibody halfinhibitory concentration (IC 50) of 0.86 ng/mL, a detection limit of 0.28 ng/mL, and a linear detection range of 0.28–2.26 ng/mL. The cross-reaction values of SLM and MLN were 100% and 74.48%, respectively. The recovery of honey samples ranged from 94.6% to 100.4%, thus indicating that this analytical method can be used for the analysis of real honey samples. In addition, immunochromatographic bands were developed for qualitative, semi-quantitative and speckle detection analysis. The critical values of SLM and MLN in honey were 40 and 40 ng/mL, respectively. The results showed that these analyses can be used for real samples, with the most stringent MRL of 40 ng/mL, for SLM and MLN. [ABSTRACT FROM AUTHOR]
- Subjects :
- *SALINOMYCIN
*HONEY
*DETECTION limit
*CROSS reactions (Immunology)
Subjects
Details
- Language :
- English
- ISSN :
- 09540105
- Volume :
- 30
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Food & Agricultural Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 139900352
- Full Text :
- https://doi.org/10.1080/09540105.2019.1649370