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Development of an immunochromatographic strip assay based on a monoclonal antibody for detection of cimaterol.
- Source :
-
Food & Agricultural Immunology . Dec2019, Vol. 30 Issue 1, p1162-1173. 12p. - Publication Year :
- 2019
-
Abstract
- An anti-cimaterol (cima) monoclonal antibody, 2H8, was prepared and used to develop an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and an immunochromatographic test strip assay for the detection of cima, a newly described β-agonist. The half-maximal inhibitory concentration of the antibody was 1.56 ng/mL, and no cross-reactivity with other β-adrenergic agonists was observed. The antibody's limit of detection was 0.416 ng/mL, and its linear range of detection was 0.416–5.868 ng/mL. The recovery rates of cima in swine urine ranged from 86% to 92.5%, and the test strip had a cut-off value of 10 ng/mL. Both ic-ELISA and the developed strip assay are effective when detecting cima in swine urine. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09540105
- Volume :
- 30
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Food & Agricultural Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 139900367
- Full Text :
- https://doi.org/10.1080/09540105.2019.1674787