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A rapid competitive ELISA assay of Okadaic acid level based on epoxy-functionalized magnetic beads.
- Source :
-
Food & Agricultural Immunology . Dec2019, Vol. 30 Issue 1, p1286-1302. 17p. - Publication Year :
- 2019
-
Abstract
- Using newly designed epoxy group activated carbon shell magnetic beads as a goat against mouse antibody (G-Mab) carrier, a competitive immunoassay was conducted on the surface of G-Mab-coated magnetic beads (G-Mab-MBs). Mouse monoclonal antibody against Okadaic acid (MAb), Okadaic acid-horseradish peroxidase conjugate (OA-HRP) and OA were simultaneously added into a reaction system. The enzyme-linked immunosorbent assay (ELISA) was performed in a 96-well microplate incorporated with magnetic bars. Detection of the OA level by using the current approach provided an excellent linear relationship in the range of 0.35ā25.0 µg Lā1 with a half-maximal inhibitory concentration IC50 of 3.27 µg Lā1 and LOD values of 0.35 µg Lā1. The shellfish matrix and other algal toxins presented in the sample extraction did not produce obvious interference. As a comparison, tests were conducted via commercial ELISA and liquid chromatography-tandem mass spectrometry (LC-MS/MS), and the corresponding analytical results demonstrated the reliability of the current protocol. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09540105
- Volume :
- 30
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Food & Agricultural Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 139900377
- Full Text :
- https://doi.org/10.1080/09540105.2019.1689231