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Characterization of the Group I and Group II antibody response against PC-KLH in normal and T15 idiotype-suppressed BALB/c mice.
- Source :
-
Immunology . Jul88, Vol. 64 Issue 3, p385-390. 6p. - Publication Year :
- 1988
-
Abstract
- The memory response of BALB/c mice to phosphocholine-keyhole limpet haemocyanin (PC-KLH) consists of two antibody populations, designated Group I and Group II, that differ in their fine specificity, as determined by hapten inhibition using phosphocholine (PC) and p-nitrophenylphos-phocholine (NPPC). It is known that Group I response is dominated by T15 idiotype-positive antibodies that utilize the VH I heavy-chain gene in combination with VK22 light-chain gene. In this paper we show that Group II serum antibodies of BALE/c mice are also highly restricted in their heterogeneity, as determined by N-terminal amino acid sequence analysis. Furthermore, we demonstrate that the Group II response is not affected by neonatally induced T15 suppression, whereas the Group I response in these mice consists of T1 5-negative antibodies. This suggests that the expression of the two antibody populations is regulated independently. Finally, we show that the isotype distributions within a fine specificity are the same in normal and T15-suppressed mice. Interestingly this is true not only for the Group II but also for the Group I antibodies. Because the isolated Group I antibodies from normal mice differ in structure from those of T15-suppressed mice, i.e. different light chains, our data indicate that the isotype distribution of these two populations is associated with their fine specificity in addition to their clonal origin. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 64
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 14008104