Electrochemiluminescence immunosensor of "signal-off" for β-amyloid detection based on dual metal-organic frameworks.

In this work, a "signal-off" sandwich electrochemiluminescence (ECL) immunosensor was fabricated for ingenious detection of Alzheimer's disease marker β-amyloid (Aβ) based on dual metal-organic frameworks (dual-MOFs), which included NH 2 -UiO-66 and MIL-101. Dual-MOFs with high porosity and plentiful functional groups can increase the biomolecules binding rate, and through the synergy with the different materials can effectively amplify the inherent properties of the material itself. Ru(bpy) 3 2+ was capsulated in NH 2 -UiO-66 as luminophor, the complex possessed steady and excellent luminescence efficiency as well as large specific surface area to load sufficient antibodies. MoS 2 quantum dots (MoS 2 QDs) combined with MIL-101 via Au–S bond. MIL-101@Au–MoS 2 QDs was used for the label of secondary antibodies (Ab 2) to quench the ECL signal of Ru(bpy) 3 2+/NH 2 -UiO-66. The amount of Ab 2 -MIL-101@Au–MoS 2 QDs bound to antigen will gradually accrue with the increase of the concentration of Aβ, which led to "signal-off" for accurate estimation of Aβ. Under optimal conditions, the developed assay for Aβ detection demonstrated a wide linear range of 10−5 ng mL−1 to 50 ng mL−1 and the detection limit was as low as 3.32 fg mL−1 (S/N = 3). The cooperation of dual-MOFs has potential as a universal strategy for quantitative analysis of other targets. Ru(bpy) 3 2+ and MoS 2 QDs simultaneously reacted with tripropylamine (TPA) to consume the co-reactant in the PBS solution, resulting in "Signal off". Image 1 • Dual-MOFs were applied to ECL signal amplification and annihilation. • A "signal-off" method for detecting Aβ was developed. • Ru(bpy) 3 2+/NH 2 -UiO-66 existed excellent ECL signal. • The quenching mechanism of MoS 2 to Ru(bpy) 3 2+ was firstly proposed. [ABSTRACT FROM AUTHOR]