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Hydroxysafflor Yellow A Inhibits TNF-α-Induced Inflammation of Human Fetal Lung Fibroblasts via NF-κB Signaling Pathway.
- Source :
-
Evidence-based Complementary & Alternative Medicine (eCAM) . 12/26/2019, p1-10. 10p. 4 Graphs. - Publication Year :
- 2019
-
Abstract
- Objective. Hydroxysafflor yellow A (HSYA), an effective ingredient of the Chinese herb Carthamus tinctorius L, attenuated bleomycin-induced pulmonary fibrosis in mice. This study is to investigate the effect of HSYA on the proliferation and inflammatory level of human fetal lung fibroblasts (MRC-5 cells) induced by tumor necrosis factor-α (TNF-α) and explore the underlying mechanisms. Methods. MRC-5 cells were treated with different concentrations of TNF-α, HSYA, or/and etanercept (ENCP, TNF-α receptor (TNFR1) antagonist, 500 ng/mL) before cell proliferation was detected. The laser confocal microscope was used to observe the role of HSYA in binding of TNF-α and its receptor. Co-immunoprecipitation was used to detect the binding of TNFR1 and TAK1-TAB2 complex. Real-time quantitative RT-PCR and western blot were used to detect the expressions of inflammation-related cytokines and proteins related with the NF-κB pathway. Luciferase reporter gene assay and chromatin coprecipitation method were used to detect the interaction between AP-1 and TGF-β1 promoter. Results. TNF-α (5 ng/mL) was used to induce inflammation and proliferation in MRC-5 cells. HSYA can partially suppress the stimulation of TNF-α on proliferation and inflammatory response of MRC-5 cells. HSYA could compete with TNF-α to bind with TNFR1 and hamper the binding of TNFR1 to TAK1-TAB2 complex. In addition, HSYA could also inhibit the activation of the NF-κB signal pathway and suppress the binding of TGF-β1 promoter with AP-1. Conclusion. Evidence in this study suggested that HSYA affects TNF-α-induced proliferation and inflammatory response of MRC-5 cells through the NF-κB/AP-1 signaling pathway, which may provide theoretical basis for HSYA treatment in pulmonary fibrosis. [ABSTRACT FROM AUTHOR]
- Subjects :
- *INFLAMMATION prevention
*CELL proliferation
*BIOLOGICAL assay
*CELL receptors
*CELLULAR signal transduction
*CYTOKINES
*FIBROBLASTS
*GENE expression
*HERBAL medicine
*INFLAMMATORY mediators
*LUNGS
*CHINESE medicine
*MICROSCOPY
*POLYMERASE chain reaction
*PULMONARY fibrosis
*TRANSCRIPTION factors
*TRANSFORMING growth factors-beta
*TUMOR necrosis factors
*WESTERN immunoblotting
*DNA-binding proteins
*ETANERCEPT
*REVERSE transcriptase polymerase chain reaction
*PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 1741427X
- Database :
- Academic Search Index
- Journal :
- Evidence-based Complementary & Alternative Medicine (eCAM)
- Publication Type :
- Academic Journal
- Accession number :
- 140844586
- Full Text :
- https://doi.org/10.1155/2019/4050327