Structure-function insights into elusive Mycobacterium tuberculosis protein Rv1916.

Tuberculosis (TB) is one of the leading causes of death worldwide. Long duration of TB therapy, results in the persistence and development of drug resistant strains of causative organism Mycobacterium tuberculosis (Mtb). Novel drug targets against persistent Mtb is an immediate need for overcoming this global menace. Isocitrate lyase (ICL), the first enzyme of glyoxylate pathway, is essential for persistent Mtb and absent in humans, hence a propitious target for drug development. Pathogenic Mtb H37Rv, have two types of ICLs - ICL1 encoded by icl (Rv0467) is well characterized and homologous to eubacterial enzyme whereas ICL2 encoded by aceA is more related to eukaryotic isocitrate lyase. To compound it, the aceA gene is split into two ORFs namely rv1915 / aceAa and rv1916 / aceAb. No translational product has been reported for the later and therefore, in vivo existence of Rv1916/ICL2 b is debatable. This study reports recombinant production of Rv1916 in heterologous host E. coli BL21 (DE3) for structure function studies. The studies categorically demonstrate that akin to Mtb ICL1, recombinant Rv1916 also possess dual ICL and methylisocitrate lyase (MICL) activities in vitro. Based on in silico analysis, a putative function linked to secondary metabolite synthesis is assigned to unique mycobacterial domain IV. • This study is the first report of Rv1916 possessing in vitro ICL and MICL activities. • 3-Nitropropionate is an uncompetitive inhibitor of both the activities. • Phylogenetic analysis suggests, evolutionary relationship between Rv1916 and ICLs of some soil bacterial species. • Domain IV of Rv1916, is hypothesized to be involved in synthesis of secondary metabolites. [ABSTRACT FROM AUTHOR]