低钙饲料联合地塞米松致大鼠骨质疏松模型的建立.

Objective To study the feasibility of establishing a rat osteoporosis model by low calcium diet combined with intramuscular dexamethasone. Methods 20 female SD rats of 3 months old were randomly divided into two groups: normal control group(normal feed + normal saline), model group(low calcium feed + dexamethasone), with 10 rats in each group. The model group received dexamethasone every three days, as intramuscular injection at the leg at a dose of 1 mg/kg, and the same amount of normal saline was injected into the blank group. Bone mineral density was measured by a dual energy X-absorption bone densitometer after four weeks of administration. Bone mineral density was measured again in the sixth week, and further evaluated by indicators such as ashes/dry weight, bone calcium, bone phosphorus, blood calcium, blood phosphorus, and bone histomorphology. Results There was no significant difference in bone mineral density between the model group and the blank group(P>0.05) at the forth week. Bone density of the model group at the sixth week was significantly lower than that in the blank group(P<0.01). The ash weight/dry weight in the bone tissue of the model group was significantly lower than that in the blank group(P<0.01). The contents of bone calcium and bone phosphorus in the model group were significantly lower than those in the blank group(P<0.01). The serum calcium concentration in the model group was significantly higher than that in the blank group(P<0.01). The concentration of phosphorus of the model group was significantly lower than that of the blank group(P<0.01). It was evident from the pathological tissue sections that the trabecular bone of the model group was incomplete and there were a large number of fat cells. Conclusion Dexamethasone + low calcium feed modeling method is feasible and can shorten the model time. [ABSTRACT FROM AUTHOR]