Back to Search
Start Over
Microsatellite Marker Discovery in the Stingless Bee Uruçu-Amarela (Melipona rufiventris Group, Hymenoptera, Meliponini) for Population Genetic Analysis.
- Source :
-
Insects (2075-4450) . Dec2019, Vol. 10 Issue 12, p450-450. 1p. - Publication Year :
- 2019
-
Abstract
- The species Melipona rufiventris Lepeletier, 1836 is a Brazilian native stingless bee that is part of a species complex known as the 'rufiventris group', making it difficult to distinguish between the different species. Populations in this group are facing a severe decline, leading to the risk of local extinction, and therefore, their conservation should be treated as a major concern. This study describes the first set of tri- and tetranucleotide microsatellite markers, using next-generation sequencing technology for use in the identification of genetic diversity and population structure in the 'rufiventris group'. A total of 16 microsatellite loci displayed polymorphism. Analysis of the whole data set (n = 50) detected 63 alleles in all loci, ranging from 2 to 7 with a mean of 3.9 alleles/locus. A genetic diversity analysis revealed high values for population differentiation estimates (FST = 0.252, RST = 0.317, and DEST = 0.284) between the Atlantic Forest, Cerrado, and Caatinga biomes. An additional evidence for genetic divergence among populations was also found in the 'rufiventris group'; these should be treated as separate conservation units or even as separate species. These microsatellite markers have demonstrated a strong potential for assessing population discrimination in this threatened stingless bee group. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 20754450
- Volume :
- 10
- Issue :
- 12
- Database :
- Academic Search Index
- Journal :
- Insects (2075-4450)
- Publication Type :
- Academic Journal
- Accession number :
- 140904524
- Full Text :
- https://doi.org/10.3390/insects10120450