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A Plasmodium falciparum C -mannosyltransferase is dispensable for parasite asexual blood stage development.
- Source :
-
Parasitology . Dec2019, Vol. 146 Issue 14, p1767-1772. 6p. - Publication Year :
- 2019
-
Abstract
- C- mannosylation was recently identified in the thrombospondin-related anonymous protein (TRAP) from Plasmodium falciparum salivary gland sporozoites. A candidate P. falciparum C -mannosyltransferase (Pf DPY-19) was demonstrated to modify thrombospondin type 1 repeat (TSR) domains in vitro , exhibiting a different acceptor specificity than their mammalian counterparts. According to the described minimal acceptor of Pf DPY19, several TSR domain-containing proteins of P. falciparum could be C -mannosylated in vivo. However, the relevance of this protein modification for the parasite viability remains unknown. In the present study, we used CRISPR/Cas9 technology to generate a Pf DPY19 null mutant, demonstrating that this glycosyltransferase is not essential for the asexual blood development of the parasite. Pf DPY19 gene disruption was not associated with a growth phenotype, not even under endoplasmic reticulum-stressing conditions that could impair protein folding. The data presented in this work strongly suggest that Pf DPY19 is unlikely to play a critical role in the asexual blood stages of the parasite, at least under in vitro conditions. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00311820
- Volume :
- 146
- Issue :
- 14
- Database :
- Academic Search Index
- Journal :
- Parasitology
- Publication Type :
- Periodical
- Accession number :
- 140922354
- Full Text :
- https://doi.org/10.1017/S0031182019001380