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Efficient ferulic acid and xylo-oligosaccharides production by a novel multi-modular bifunctional xylanase/feruloyl esterase using agricultural residues as substrates.
- Source :
-
Bioresource Technology . Feb2020, Vol. 297, pN.PAG-N.PAG. 1p. - Publication Year :
- 2020
-
Abstract
- • A novel bifunctional xylanase/feruloyl esterase (rXyn10A/Fae1A) was obtained. • The highest ferulic acid yield (7.31 mg/g substrate) was produced by rXyn10A/Fae1A. • Inter-domain synergism of rXyn10A/Fae1A is essential for the release of ferulic acid. • Recombinant E. coli was constructed and secreted the bifunctional enzyme out of cells. • Co-production of ferulic acid and XOSs during the cultivation of recombinant E. coli. Liberating high value-added compounds ferulic acid (FA) and xylo-oligosaccharides (XOSs) from agricultural residues is a promising strategy for the utilization of lignocellulose. In this study, a bifunctional xylanase/feruloyl esterase from bacterial consortium EMSD5 was heterogeneously expressed in Escherichia coli. Depending on the inter-domain synergism of the recombinant enzyme rXyn10A/Fae1A, high yields of FA (2.78, 1.82, 1.15 and 7.31 mg/g substrate, respectively) were obtained from 20 mg in-soluble wheat arabinoxylan, de-starched wheat bran, ultrafine-grinding corn stover and steam-exploded corncob. Meanwhile, 3.210, 1.235, 1.215 and 0.823 mg xylose/XOSs were also released. For cost-saving enzyme production, we firstly constructed a recombinant E. coli , which could secrete the bifunctional xylanase/feruloyl esterase out of cells. When the recombinant E. coli was cultured in medium containing 200 mg de-starched wheat bran, 474 μg FA and 18.2 mg xylose/XOSs were also detected. Hence, rXyn10A/Fae1A and the recombinant strain showed great applied potential for FA and XOSs production. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09608524
- Volume :
- 297
- Database :
- Academic Search Index
- Journal :
- Bioresource Technology
- Publication Type :
- Academic Journal
- Accession number :
- 140957844
- Full Text :
- https://doi.org/10.1016/j.biortech.2019.122487