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Inhibition of Wnt-2-mediated signaling induces programmed cell death in non-small-cell lung cancer cells.
- Source :
-
Oncogene . 8/12/2004, Vol. 23 Issue 36, p6170-6174. 5p. - Publication Year :
- 2004
-
Abstract
- In this report, we have demonstrated that Wnt-2 protein is overexpressed in freshly resected human non-small-cell lung cancer (NSCLC) tissues. We have also developed a monoclonal antibody against the N-terminus of human Wnt-2 protein. This monoclonal antibody induces apoptosis in human NSCLC cell lines that overexpress Wnt-2 protein. Incubation of this antibody with normal human airway cells lacking Wnt-2 expression does not induce apoptosis. Wnt-2 signaling blockade by the anti-Wnt-2 antibody is confirmed by downregulation of cytosolic ß-catenin and reduction in TCF-dependent transcriptional activity (TOPFLASH assay). In addition, Wnt-2-specific small interfering RNA (siRNA) treatment in the NSCLC cell line A549 also downregulated cytosolic ß-catenin and induced apoptosis. Moreover, downregulation of an inhibitor of apoptosis family protein, Survivin, was noticed both in the Wnt-2 antibody- and siRNA-treated NSCLC cells, suggesting that inhibition of Wnt-2-mediated signaling induces apoptosis through inactivating Survinin.Oncogene (2004) 23, 6170-6174. doi:10.1038/sj.onc.1207844 Published online 21 June 2004 [ABSTRACT FROM AUTHOR]
- Subjects :
- *APOPTOSIS
*CELL death
*TISSUES
*CELLS
*IMMUNOGLOBULINS
*MOLECULAR cloning
Subjects
Details
- Language :
- English
- ISSN :
- 09509232
- Volume :
- 23
- Issue :
- 36
- Database :
- Academic Search Index
- Journal :
- Oncogene
- Publication Type :
- Academic Journal
- Accession number :
- 14097062
- Full Text :
- https://doi.org/10.1038/sj.onc.1207844