The establishment of CMV antigen-specific enzyme-linked immunospot assay among Chinese: A pilot study.

• First study on detecting CMV antigen-specific cellular immune response in China. • Significant difference in SFCs can be found among different infection state groups. • The performance of pp65 antigen is better than IE-1. The prevalence of cytomegalovirus in China is high and the infection could result in disastrous consequences. Cellular immunity is the main mechanism for virus controlling. We explored the performance of cytomegalovirus antigen-specific enzyme-linked immunospot assay in patients with different infection states in endemic area. Twenty-eight patients with reliable results were included in statistical analysis. Peripheral blood mononuclear cells were extracted and were stimulated by cytomegalovirus phosphoprotein 65 or immediate early protein-1 antigen respectively. Spot forming cells (SFCs) were counted to evaluate the cellular immune response elicited by antigens. Spots could be clearly displayed, and evenly dispersed with a clean background. The numbers of SFCs were 0 [0–0], 426 [210–601] and 230 [57–513] for uninfected individuals (n = 7), latently infected patients (n = 11) and actively infected patients (n = 10) respectively, which were statistically different. The number of SFCs stimulated by phosphoprotein 65 was significantly higher than that by immediate early protein-1. The number of SFCs was significantly different among patients with different infection state. The stimulatory effect of phosphoprotein 65 was better than that of immediate early protein-1. [ABSTRACT FROM AUTHOR]