Back to Search
Start Over
Discovery and application of methionine sulfoxide reductase B for preparation of (S)-sulfoxides through kinetic resolution.
- Source :
-
Catalysis Communications . Mar2020, Vol. 136, pN.PAG-N.PAG. 1p. - Publication Year :
- 2020
-
Abstract
- Here we report a methionine sulfoxide reductase B (MsrB) enzymatic system for the preparation of (S)-sulfoxides through kinetic resolution of racemic (rac) sulfoxides. Eight MsrB homologue recombinant proteins were expressed and their activities on asymmetric reduction of rac -sulfoxides were analyzed. Among these MsrB homologue proteins, one protein from Acidovorax species showed good activity and enantioselectivity towards several aryl-alkyl sulfoxides. The (S)-sulfoxides were prepared with 93–98% enantiomeric excess through kinetic resolution at initial substrate concentration up to 50 mM. The establishment of MsrB catalytic kinetic resolution system provides a new efficient green strategy for preparation of (S)-sulfoxides. Unlabelled Image • MsrB was firstly applied for (S)-sulfoxides preparation through kinetic resolution. • MsrB from Acidovorax species showed good activity and enantioselectivity towards several rac -sulfoxides. • (S)-sulfoxides were prepared with 93–98% of ee at substrate concentration up to 50 mM. [ABSTRACT FROM AUTHOR]
- Subjects :
- *METHIONINE sulfoxide reductase
*KINETIC resolution
*RECOMBINANT proteins
Subjects
Details
- Language :
- English
- ISSN :
- 15667367
- Volume :
- 136
- Database :
- Academic Search Index
- Journal :
- Catalysis Communications
- Publication Type :
- Academic Journal
- Accession number :
- 141279544
- Full Text :
- https://doi.org/10.1016/j.catcom.2019.105908