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Effect of gga-miR-155 on cell proliferation, apoptosis and invasion of Marek's disease virus (MDV) transformed cell line MSB1 by targeting RORA.
- Source :
-
BMC Veterinary Research . 1/28/2020, Vol. 16 Issue 1, p1-9. 9p. - Publication Year :
- 2020
-
Abstract
- Background: Marek's disease (MD) is caused by the oncogenic Marek's disease virus (MDV), and is a highly contagious avian infection with a complex underlying pathology that involves lymphoproliferative neoplasm formation. MicroRNAs (miRNAs) act as oncogenes or tumor suppressors in most cancers. The gga-miR-155 is downregulated in the MDV-infected chicken tissues or lymphocyte lines, although its exact role in tumorigenesis remains unclear. The aim of this study was to analyze the effects of gga-miR-155 on the proliferation, apoptosis and invasiveness of an MDV-transformed lymphocyte line MSB1 and elucidate the underlying mechanisms. Results: The expression level of gga-miR-155 was manipulated in MSB1 cells using specific mimics and inhibitors. While overexpression of gga-miR-155 increased proliferation, decreased the proportion of G1 phase cells relative to that in S and G2 phases, reduced apoptosis rates and increased invasiveness. However, its downregulation had the opposite effects. Furthermore, gga-miR-155 directly targeted the RORA gene and downregulated its expression in the MSB1 cells. Conclusion: The gga-miR-155 promotes the proliferation and invasiveness of the MDV-transformed lymphocyte line MSB1 and inhibits apoptosis by targeting the RORA gene. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 17466148
- Volume :
- 16
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- BMC Veterinary Research
- Publication Type :
- Academic Journal
- Accession number :
- 141432039
- Full Text :
- https://doi.org/10.1186/s12917-020-2239-4